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Detection of Escherichia coli in wastewater based on enzyme immunoassay

XI Haiyan, CAI Qiang, HE Miao, SHI Hanchang

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 3,   Pages 381-384 doi: 10.1007/s11783-007-0065-6

Abstract: This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horse radish peroxidase (HRP)- conjugate, and anti-HPR antibody and pretreatment of were studied. Those results showed that the linear range of detection for was 10 cfu/mL– 6 × 10 cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost.

Keywords： conventional     sandwich     pretreatment     enzyme-linked immunosorbent     detection    

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italic>rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichiacoli under an aerobic condition

Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 2,   Pages 152-157 doi: 10.1007/s11705-012-1287-0

Abstract: The role of on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose-enriched culture conditions. The experimental results showed that while the wild type cells exhibited an overflow metabolism effect, the -deleting mutation alleviated this effect with the significant suppression of acetate accumulation under a high glucose condition. This gene deletion also induced the twofold upregulation of and one-tenth downregulation of , respectively. The overflow metabolism effect was confirmed to be recovered by re-introducing gene into the mutant. These results demonstrated changed the central carbon metabolism toward acetate overflow through dehydrogenation of pyruvate and reduction of TCA cycle activity.

Keywords： Escherichia coli     rpoS     aerobic and glucose-enriched culture     overflow    

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Enhanced disinfection of Escherichia coli and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0854-x

Abstract: A novel photocatalytic Ag-Cu-TiO nanowire membrane was fabricated. Bacteria and virus disinfection was improved by co-depositing Ag and Cu onto membrane. Synergetic photocatalytic effects and free metal ions of Ag and Cu contribute to disinfection. 7.68 log removal of and 4.02 log removal of bacteriophage MS2 were achieved. Titanium dioxide (TiO ) is a widely used photocatalyst that has been demonstrated for microorganism disinfection in drinking water. In this study, a new material with a novel structure, silver and copper loaded TiO nanowire membrane (Cu-Ag-TiO ) was prepared and evaluated for its efficiency to inactivate and bacteriophage MS2. Enhanced photo-activated bactericidal and virucidal activities were obtained by the Cu-Ag-TiO membrane than by the TiO , Ag-TiO and Cu-TiO membranes under both dark and UV light illumination. The better performance was attributed to the synergies of enhanced membrane photoactivity by loading silver and copper on the membrane and the synergistic effect between the free silver and copper ions in water. At the end of a 30 min test of dead-end filtration under 254 nm UV irradiation, the Cu-Ag-TiO membrane was able to obtain an removal of 7.68 log and bacteriophage MS2 removal of 4.02 log, which have met the US EPA standard. The free metal ions coming off the membrane have concentrations of less than 10 ppb in the water effluent, far below the US EPA maximum contaminant level for silver and copper ions in drinking water. Therefore, the photo-activated disinfection by the Cu-Ag-TiO membrane is a viable technique for meeting drinking water treatment standards of microbiological water purifiers.

Keywords： Photo-activated disinfection     Titanium dioxide     Nanowire membrane     Silver     Copper    

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Characteristics of two transferable aminoglycoside resistance plasmids in

Chengjun Pu, Xiaoyan Gong, Ying Sun

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1119-2

Abstract:

pRKZ3 is a non-conjugative IncQ plasmid, while pKANJ7 is a conjugative IncX plasmid.

The optimal mating time of pKANJ7 varied under different conditions.

Both of the two transferable ARPs had little impact on the growth of their hosts.

A relatively high level of fitness cost was observed for pKANJ7.

The fitness cost of ARPs depended on their hosts.

Keywords： Escherichia coli     Characteristics     Aminoglycoside resistance plasmids     Transfer     Persistence    

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Analysis of antibiotic resistance of

Yangyang Yu, Xiaolin Zhu, Guanlan Wu, Chengzhi Wang, Xing Yuan

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1123-6

Abstract: listitem>

There was a seasonal difference in antibiotic resistance of E. coli

The E. coli in the Yitong River was found

Keywords： Yitong River     Coliform bacteria     Antibiotic resistance     Escherichia coli    

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Biomimetic Macrophage–Fe3O4@PLGA Particle-Triggered Intelligent Catalysis for Killing Multidrug-Resistant Escherichiacoli

Jieni Fu,Xiangmei Liu,Zhaoyang Li,Yufeng Zheng,Yu Zhang,Hui Jiang,Yanqin Liang,Shengli Zhu,Zhenduo Cui,Shuilin Wu,

Engineering doi: 10.1016/j.eng.2023.05.022

Abstract: Infections with multidrug-resistant (MRD) Gram-negative bacteria, such as MRD Escherichia coli (E. coli), remain a challenge due to the lack of safe antibiotics and high fatality ratessub>O4@poly(lactic-co-glycolic acid) (PLGA) nanoparticles) for killing MDR E. colii.e., the intelligent catalysis particles) exhibit selective biocatalysis activity toward MDR E. coliThe highly toxic •OH and antimicrobial peptides are shown to combat with MDR E. coli, such that

Keywords： Multidrug-resistant Escherichia coli     Macrophage–Fe3O4@PLGA particles     Biomimetic intelligent catalysis    

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Genetic and Functional Differences of Escherichia coli Strains from Colorectal Cancer Mucosal Article

Yuxiao Chang, Xiang Li, Lei Ding, Chao Yang, Zhiyuan Pan, Ni Han, Yujun Cui, Fachao Zhi, Ruifu Yang, Hong Gao, Yujing Bi

Engineering 2022, Volume 16, Issue 9,   Pages 210-219 doi: 10.1016/j.eng.2021.03.028

Abstract: Here, we used culturomics to isolate CRC mucosal samples and selected 158 Escherichia coli strains

Keywords： Colorectal cancer     Gut microbiota     Culturomics     Escherichia coli     Strain level    

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Effect of ultraviolet irradiation and chlorination on ampicillin-resistant

Yuchen PANG,Jingjing HUANG,Jinying XI,Hongying HU,Yun ZHU

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 3,   Pages 522-530 doi: 10.1007/s11783-015-0779-9

Abstract: Antibiotic resistance is a serious public health risk that may spread via potable and reclaimed water. Effective disinfection is important for inactivation of antibiotic-resistant bacteria and disruption of antibiotic resistance genes. Ampicillin is a widely prescribed antibiotic but its effectiveness is increasingly undermined by resistance. In this study, changes in ampicillin resistance for ( ) CGMCC 1.1595 were analyzed after exposure to different doses of ultraviolet (UV) or chlorine, and damage incurred by the plasmid encoding ampicillin resistance gene was assessed. We reported a greater stability in ampicillin-resistant CGMCC 1.1595 after UV irradiation or chlorination when compared with previously published data for other strains. UV irradiation and chlorination led to a shift in the mortality frequency distributions of ampicillin-resistant when subsequently exposed to ampicillin. The ampicillin hemi-inhibitory concentration (IC ) without disinfection was 3800 mg·L , and an increment was observed after UV irradiation or chlorination. The IC of ampicillin-resistant was 1.5-fold higher at a UV dose of 40 mJ·cm , and was 1.4-fold higher when exposed to 2.0 mg·L chlorine. These results indicate that UV irradiation and chlorination can potentially increase the risk of selection for strains with high ampicillin resistance. There was no evident damage to after 1–10 mg Cl ·L chlorination, while a UV dose of 80 mJ·cm yielded a damage ratio for of approximately 1.2-log. Therefore, high UV doses are required for effective disruption of antibiotic resistance genes in bacteria.

Keywords： antibiotic resistance     Escherichia coli     ampicillin resistance gene     ultraviolet irradiation     chlorination    

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Transmission of Carbapenem Resistance Between Human and Animal NDM-Positive Escherichia coli Strains Article

Yingbo Shen, Fupin Hu, Yongqiang Wang, Dandan Yin, Lu Yang, Yiqiang Chen, Chunyan Xu, Jiyun Li, Junyao Jiang, Xueyang Wang, Yulin Fu, Dongyan Shao, Dejun Liu, Tengfei Ma, Chang Cai, Zhangqi Shen, Shaolin Wang, Juan Li, Rong Zhang, Yuebin Ke, Congming Wu, Jianzhong Shen, Timothy R. Walsh, Yang Wang

Engineering 2022, Volume 15, Issue 8,   Pages 24-33 doi: 10.1016/j.eng.2021.07.030

Abstract: text-align: justify;">Although carbapenem use is prohibited in animals in China, carbapenem-resistant Escherichiacoli (CREC), especially New Delhi metallo-β-lactamase (NDM)-producing strains, are widelyOverall, 631/29799 (2.1%) clinical Escherichia coli (E. coli) isolates were identifiedAmong all the Chinese NDM-positive E. coli (n = 463) available at the National Center

Keywords： Carbapenem resistance     Escherichia coli     blaNDM-5     One Health     Antimicrobial    

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Deposition of copper nanoparticles on multiwalled carbon nanotubes modified with poly (acrylic acid) and their antimicrobial application in water treatment

Li SHENG,Shuhang HUANG,Minghao SUI,Lingdian ZHANG,Lei SHE,Yong CHEN

Frontiers of Environmental Science & Engineering 2015, Volume 9, Issue 4,   Pages 625-633 doi: 10.1007/s11783-014-0711-8

Abstract: A novel hybrid material, Cu-PAA/MWCNTs (copper nanoparticles deposited multiwalled carbon nanotubes with poly (acrylic acid) as dispersant, was prepared and expected to obtain a more effective and well-dispersed disinfection material for water treatment. X-ray energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), the X-ray fluorescence (XRF), X-ray photoelectron spectra (XPS), Fourier transform infrared spectra (FT-IR), Raman spectroscopy, and thermal gravimetric analyzer (TGA) were used to characterize the Cu-PAA/MWCNTs. ( ) was employed as the target bacteria. The cell viability determination and fluorescence imaging results demonstrated that Cu-PAA/MWCNTs possessed strong antimicrobial ability on . The deposited Cu was suggested to play an important role in the antimicrobial action of Cu-PAA/MWCNTs.

Keywords： multiwalled carbon nanotubes     copper nanoparticles     antimicrobial activity     Escherichia coli (    

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Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 6, doi: 10.1007/s11783-016-0884-4

Abstract: Flow cytometry based on nucleic acid stains and CTC was established and optimized. Membrane of is more resistant to chlorine/chloramine than . The metabolic activity of bacteria persisted after the cytomembranewas damaged. showed more resistance to UV irradiation than by FCM. MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Flow cytometry (FCM) has been widely used in multi-parametric assessment of cells in various research fields, especially in environmental sciences. This study detected the metabolic activity of and by using an FCM method based on 5-cyano-2,3-ditolyltetrazolium chloride (CTC); the accuracy of this method was enhanced by adding SYTO 9 and 10% R2A broth. The disinfection effects of chlorine, chloramine, and UV were subsequently evaluated by FCM methods. Chlorine demonstrated stronger and faster destructive effects on cytomembrane than chloramine, and nucleic acids decomposed afterwards. The metabolic activity of the bacteria persisted after the cytomembranewas damaged as detected using CTC. Low-pressure (LP) UV or medium-pressure (MP) UV treatments exerted no significant effects on membrane permeability. The metabolic activity of the bacteria decreased with increasing UV dosage, and MP-UV was a stronger inhibitor of metabolic activity than LP-UV. Furthermore, the membrane of Gram-positive was more resistant to chlorine/chloramine than that of Gram-negative . In addition, showed higher resistance to UV irradiation than .

Keywords： Flow cytometry     Escherichia coli     Staphylococcusaureus     UV     CTC     SYTO 9    

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A One-Health Sampling Strategy to Explore the Dissemination and Relationship Between Colistin Resistance in Human, Animal, and Environmental Sectors in Laos Article

Yuqing Zhou, Refath Farzana, Somsavanh Sihalath, Sayaphet Rattanavong, Manivanh Vongsouvath, Mayfong Mayxay, Kirsty Sands, Paul N. Newton, David A.B. Dance, Brekhna Hassan, Timothy R. Walsh

Engineering 2022, Volume 15, Issue 8,   Pages 45-56 doi: 10.1016/j.eng.2022.01.013

Abstract: A total of 238 Escherichia coli (E. coli) isolated from nonduplicative samples, consistingof 98 MCR-positive E. coli (MCRPEC) ("mcr" denotes the gene encodingquot;MCR" denotes the subsequent protein encoded by mcr) and 140 MCRnegative E. coli

Keywords： Colistin resistance     mcr     Escherichia coli Laos     Horizontal transmission    

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Mobile CRISPR-Cas9 based anti-phage system in E. coli

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 8,   Pages 1281-1289 doi: 10.1007/s11705-022-2141-7

Abstract: Escherichia coli is one of the most important microbial cell factories, but infection by bacteriophagesHere, we developed a mobile CRISPR-Cas9 based anti-phage system for bacteriophages defense in E. coliFor preventing phage infection in E. coli, the pGM2 decreased the phage infection rate to 0.1%These results support the potential application of MCBAS in E. coli cell factories to defend against

Keywords： phage infections     anti-phage     CRISPR-Cas9     conjugative transfer     synthetic biology    

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Transport of bacterial cell (

Wei Fan, Qi Li, Mingxin Huo, Xiaoyu Wang, Shanshan Lin

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 4, doi: 10.1007/s11783-020-1242-0

Abstract: . • E. coli cells within different recharge water displayed different spreading risks.The dwelling process in recharge pond imposed physiologic stress on Escherichia coli (E. coli) cells,in all three types of investigated recharge water resources and the cultivation of E. coli under varyingThe E. coli cells in rainfall recharge water displayed the highest mobility (least retention), followedThe findings of this study contribute to an improved understanding of E. coli transport in actual AGR

Keywords： Artificial groundwater recharge     E. coli     Transport     Simulated column experiments     Modeling    

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Title Author Date Type Operation

Detection of Escherichia coli in wastewater based on enzyme immunoassay

XI Haiyan, CAI Qiang, HE Miao, SHI Hanchang

Journal Article

italic>rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichiacoli under an aerobic condition

Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA

Journal Article

Enhanced disinfection of Escherichia coli and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

Journal Article

Characteristics of two transferable aminoglycoside resistance plasmids in

Chengjun Pu, Xiaoyan Gong, Ying Sun

Journal Article

Analysis of antibiotic resistance of

Yangyang Yu, Xiaolin Zhu, Guanlan Wu, Chengzhi Wang, Xing Yuan

Journal Article

Biomimetic Macrophage–Fe3O4@PLGA Particle-Triggered Intelligent Catalysis for Killing Multidrug-Resistant Escherichiacoli

Jieni Fu,Xiangmei Liu,Zhaoyang Li,Yufeng Zheng,Yu Zhang,Hui Jiang,Yanqin Liang,Shengli Zhu,Zhenduo Cui,Shuilin Wu,

Journal Article

Genetic and Functional Differences of Escherichia coli Strains from Colorectal Cancer Mucosal

Yuxiao Chang, Xiang Li, Lei Ding, Chao Yang, Zhiyuan Pan, Ni Han, Yujun Cui, Fachao Zhi, Ruifu Yang, Hong Gao, Yujing Bi

Journal Article

Effect of ultraviolet irradiation and chlorination on ampicillin-resistant

Yuchen PANG,Jingjing HUANG,Jinying XI,Hongying HU,Yun ZHU

Journal Article

Transmission of Carbapenem Resistance Between Human and Animal NDM-Positive Escherichia coli Strains

Yingbo Shen, Fupin Hu, Yongqiang Wang, Dandan Yin, Lu Yang, Yiqiang Chen, Chunyan Xu, Jiyun Li, Junyao Jiang, Xueyang Wang, Yulin Fu, Dongyan Shao, Dejun Liu, Tengfei Ma, Chang Cai, Zhangqi Shen, Shaolin Wang, Juan Li, Rong Zhang, Yuebin Ke, Congming Wu, Jianzhong Shen, Timothy R. Walsh, Yang Wang

Journal Article

Deposition of copper nanoparticles on multiwalled carbon nanotubes modified with poly (acrylic acid) and their antimicrobial application in water treatment

Li SHENG,Shuhang HUANG,Minghao SUI,Lingdian ZHANG,Lei SHE,Yong CHEN

Journal Article

Flow cytometric assessment of the effects of chlorine, chloramine, and UV on bacteria by using nucleic acid stains and 5-cyano-2,3-ditolyltetrazolium chloride

Xuebiao Nie, Wenjun Liu, Mo Chen, Minmin Liu, Lu Ao

Journal Article

A One-Health Sampling Strategy to Explore the Dissemination and Relationship Between Colistin Resistance in Human, Animal, and Environmental Sectors in Laos

Yuqing Zhou, Refath Farzana, Somsavanh Sihalath, Sayaphet Rattanavong, Manivanh Vongsouvath, Mayfong Mayxay, Kirsty Sands, Paul N. Newton, David A.B. Dance, Brekhna Hassan, Timothy R. Walsh

Journal Article

Wang Yang: Research on the Transmission of Carbapenem Resistant Escherichia Coli Between Humans and Animals

17 Oct 2022

Conference Videos

Mobile CRISPR-Cas9 based anti-phage system in E. coli

Journal Article

Transport of bacterial cell (

Wei Fan, Qi Li, Mingxin Huo, Xiaoyu Wang, Shanshan Lin

Journal Article